MiR-148a-3p Regulates Stem Cell Osteogenic Differentiation and Enamel Development by Targeting Runt-Related Transcription Factor 2 and E-cadherin <i>via</i> the Wnt1/β-catenin Signaling Pathway

We aimed to evaluate the regulatory effects of miR-148a-3p on stem cell osteogenic differentiation and enamel development by targeting runt-related transcription factor 2 (RUNX2) E-cadherin, respectively. TargetScan software was utilized predict binding sites between marker gene RUNX2 or E-cadherin. The changes in expression during epidermal cells were detected. After transfection with mimics inhibitor, induced towards differentiation, measured. regulated determined. liposome-mediated ameloblast development. Cell proliferation apoptosis abilities tested using methyl thiazolyl tetrazolium assay flow cytometry, detected RT-PCR. protein expressions Wnt1, β-catenin, E-cadherin measured Western blotting. Epidermal differentiated into osteoblasts through induction culture. On 5, 12, 15 30 d, gradually epithelial aggregation depression, mesenchymal aggregation, dentin secretion. transfection, compared negative control (NC) group, viability miR-148-3p group significantly decreased (P<0.05), rate increased (P<0.01). inhibitor while reduced dual-luciferase reporter showed that targeted Wnt1. Compared NC rose (P<0.001), levels decreased. increased. MiR-148a-3p is highly expressed regulates respectively via Wnt1/β-catenin pathway, which plays an important role